Research Paper Advance Articles

Figure 7. Labeling of Sqstm1 and cathepsin D in the retinal GCL and INL of young untreated, vehicle-treated and MTK-treated old mice. Positive labeling was analyzed using ImageJ. Sqstm1 particle area in the (A) GCL (area per cell) and (B) INL (per 0.01 mm²) and (C) representative images of Sqstm1 labeling (red) in the GCL of young untreated, vehicle-treated and MTK-treated old mice. Cathepsin D-positive area in the (D) GCL (area per cell) and (E) INL (0.01 mm²), and (F) representative images of cathepsin D labeling (red) in the GCL of young untreated, vehicle-treated and MTK-treated old mice. Scale bar images = 50 µm; scale bar images = 10 µm. The data are represented as bar graphs and scatter plots ± SDs, n = 3–5. Two-way ANOVA (main factors: group and sex) followed by a Dunnett multiple comparison test. ^#p < 0.05 young control vs. old vehicle-treated; ^**p < 0.01 old MTK-treated vs. old vehicle-treated; ^$p < 0.05 female vs. male.