Research Paper Advance Articles

Figure 2. Astrocytes and microglia were present in the retinas of young untreated and vehicle- and MTK-treated old mice. Astrocytes were visualized by GFAP labeling and analyzed for the (A) positive GFAP area per image in % by ImageJ. (B) Representative images of GFAP-labeled (green) retinas from young untreated, vehicle-treated and MTK-treated old mice. (C) Microglia count and (D) representative Iba1 labeling (white) in the superficial retinal layers of young untreated, vehicle-treated and MTK-treated old mice. (E) Microglia count and (F) representative Iba1 labeling in the deep retinal layers of young untreated, vehicle-treated and MTK-treated old mice. Scale bar = 100 µm. The data are represented as bar graphs and scatter plots ± SDs, n = 3–11. Two-way ANOVA (main factors: group and sex) followed by a Dunnett multiple comparison test. ^####p < 0.0001 for the young control vs. the old vehicle-treated group; ^***p < 0.001 and ^*p < 0.05 for the old MTK-treated group vs. the old vehicle-treated group; ^$$p < 0.01 for the female vs. male group.