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Figure 5. Alignment on mammalian OAT1 protein sequences. Mouse, rat, dog, and cat OAT1 protein sequences are compared to their human orthologous sequence. Dogs and cats share 91.3% and 90.9% OAT1 protein sequence identities with humans, respectively. “Cov” indicates the percentage of amino acids covered in the alignment, and “pid” indicates protein sequence identity compared to humans, consensus denotes consensus out of 80% of the sequences from the group. Red asterisks indicate the five glycosylation sites on the first extracellular loop, which is delineated by the two upward red arrows in positions 39 and 125, respectively. Humans share the same hydrophilic aspartate (D) with cats and dogs (marked by the 2^nd red arrow), but not rodents. Dog and cat’s OAT1 proteins are computationally predicted.