Research Paper Advance Articles

Figure 6. Arg-ii^-/- mice reveal preserved microvascular density in aging skeletal muscle. (A) Representative confocal images showing CD31 staining (red; endothelial cells marker) in male tibialis of young and old male wild type (wt) and arg-ii^-/- (ko) mice. DAPI (blue) is used to stain the nuclei. The graph reports the quantification of CD31 signal in the area (mm²). Scale bar: 50 µm. (B to D) Representative confocal images and relative quantification for CD31 (red) and DAPI (blue) in tibialis from young and old female wt and ko mice (B), and in soleus of young and old male (C) and female (D) wt and ko mice. Scale bar: 50 µm. One-way ANOVA was applied with n = 3 mice per group. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 between the groups.