Research Paper Advance Articles

Figure 3. Arg-ii^-/- mice reveal reduced senescent cells in aging skeletal muscles. (A) Representative confocal immunofluorescence staining of DAPI (blue) and anti-p16 (red) in tibialis from young and old wild type (wt) and arg-ii^-/- (ko) male mice. Scale bar: 50 µm. (B) Representative image of old male mouse tibialis stained with DAPI (blue), anti-p16 (red), and anti-dystrophin (yellow, marker of sarcolemma). Negative control staining without the two primary antibodies. Scale bar: 20 µm. (C, D) Quantification of p16⁺ nuclei (senescent cells) expressed as the percentage of p16⁺ nuclei/total number of nuclei in the mm² area. One-way ANOVA test was applied. n = 3–4 mice per group. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001 between the indicated groups.