Research Paper Advance Articles

Figure 7. MDPL MSCs senescence and telomere shortening. (A) The histogram shows the average percentage of β-galactosidase-positive cells in WT and MDPL mesenchymal stem cells. (B) Representative image of senescence-associated β-galactosidase assay. A greater amount of intensely positive blue cells is displayed in MDPL-MSCs than in WT controls. Arrows indicated altered cell shape. Scale bar = 200 µm. Experiments were carried in triplicates; 100 nuclei were counted per individual. Error bars indicate the standard deviation ± standard error of the mean (SEM), ^*p < 0.1, ^**p < 0.01. (C) Relative telomere length was evaluated by real-time q-PCR in MSCs MDPL and WT. Experiments were carried in triplicates. Error bars indicate the standard deviation ± standard error of the mean (SEM). ^***p < 0.001, ^****p < 0.0001 by one-way ANOVA test.