Research Paper Advance Articles
When do the pathological signs become evident? Study of human mesenchymal stem cells in MDPL syndrome
Figure 5. MSCs altered nuclear morphology analysis. (A) Histogram representing the percentage of aberrant nuclear morphology. (B) A representative image of MSCs WT and MDPL obtained by immunofluorescence for anti-Lamin A/C antibody (red). (C) Percentage of micronuclei (%MN) and percentage of Lamin B1 positive (% Lamin B1+) MN in MSCs MDPL and WT. (D) Representative immunofluorescence images showing the presence of micronuclei (MN) positive (arrow yellow) or negative (arrow white) for anti-Lamin B1 antibody (red) in MDPL cells. Hoechst nuclear staining (blue). Scale bar 100 µm. The WT data are derived from the mean of the values obtained from the two healthy control samples. Experiments were carried out in triplicates; 300 nuclei were counted per individual. Error bars indicate the standard deviation ± standard error of the mean (SEM). *p < 0.1, **p < 0.01 by one-way ANOVA test.