Research Paper Volume 16, Issue 21 pp 13252—13270

Figure 4. Model of IL1β-induced senescence in chondrocytes. (A) Schematic workflow of IL1β-induced senescence in chondrocytes. (B) Representative pictures of SA-β-Gal staining in non-treated (NT) and IL1β-treated human chondrocytes (left panel; bars: 50 µm) and percentage of SA-β-Gal positive cells (right panel) (n = 7, 8). (C) Representative pictures of chondrocytes with γH2AX foci (red) in nuclei (blue). (Left panel; bars: 100 µm). Percentage of chondrocytes with γH2AX foci (n = 5) and quantification of nucleus surface (n = 30) (right panels). (D) Relative expression of Cyclin-Dependent Kinase Inhibitors in chondrocytes (n = 6, 7). (E) Protein secretion in supernatants of IL1β-treated chondrocytes quantified by ELISA (n = 6). Statistical analysis used the Mann-Whitney test for all panels except for the nucleus surface graph where an unpaired t-test was used. ^*p < 0.05, ^**p < 0.01, ^****p < 0.0001.