Research Paper Volume 16, Issue 21 pp 13237—13251

Figure 2. Blood glucose changes after thermotherapy. Percent change of blood glucose levels after an ip injection of 1 IU/kg bw of insulin at t = 0 minutes (A). The AUC was calculated for the duration of the ITT and compared temperature effects in male (B) and female (C) mice. Percent change of blood glucose levels after an ip injection of 2 g/kg bw of glucose at t = 0 minutes (D). The AUC was calculated for both male (E) and female (F) mice. Fed (G) and fasting (H) blood glucose levels were determined prior to ip injection of insulin and glucose, respectively. The number of animals is inset on each bar graph. A two-way ANOVA factor analysis with Sidak’s post hoc was used to determine significant blood glucose changes due to thermotherapy across time intervals. A two-tailed t-test was used to determine blood glucose changes within a genotype and sex. ^*p < 0.05, ^**p < 0.01, ^****p < 0.0001.