Research Paper Volume 16, Issue 20 pp 13039—13075

Figure 9. In lens fiber cells, EphA2-pS898 signal is localized to the cell membrane of mature fibers. Lens sections in the cross orientation from 4-month-old control and ephrin-A5^-/- mice were stained with EphA2-pS898 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). E denotes the epithelial cells, and images of the fiber cells were taken in sequence along similar areas of the lens. EphA2-pS898 staining signals were enriched in mature lens fiber cells (asterisks), and these signals appear to be all around the cell membrane without preference between the broad and short sides of fibers. The staining signal was comparable between control and ephrin-A5^-/- lenses. Scale bar, 20 μm.