Research Paper Volume 16, Issue 20 pp 13039—13075

Figure 8. Non-canonically active EphA2-pS898 protein is enriched in equatorial lens epithelial cells. Longitudinal lens sections from 4-month-old control and ephrin-A5^-/- mice were stained with EphA2-pS898 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). Images of the equator region were taken in sequence along similar areas of the lens. Mature fiber cells with strong EphA2-pS898 cannot be seen in these images of the peripheral fibers. EphA2-pS898 signal was found to weakly stain anterior epithelial cells, and there was increased signal in equatorial epithelial cells (arrows). There are no obvious differences between control and ephrin-A5^-/- lenses. Scale bar, 20 μm.