Research Paper Volume 16, Issue 20 pp 13039—13075

Figure 7. Non-canonically activated EphA2-pS898 protein is localized in epithelial cells and mature fiber cells. Longitudinal lens sections from 6-week-old, 4-month-old, and 8-month-old ephrin-A5^+/+ and ephrin-A5^-/- mice were stained with EphA2-pS898 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). Fluorescence of EphA2-pS898 was found within the epithelial cells (arrows) and as a band within mature fiber cells in the lens cortex (asterisks). The staining pattern remained consistent between age-matched control and ephrin-A5^-/- lenses. There was a noticeable narrowing and intensification of the EphA2-pS898 band of signal in the cortical fiber cells region with age, which is likely due to compression of fiber cells as the lens continues to grow. Scale bar, 50 μm.