Canonical ligand-dependent and non-canonical ligand-independent EphA2 signaling in the eye lens of wild-type, knockout, and aging mice

Jenna L. Horner; Michael P. Vu; Jackson T. Clark; Isaiah J. Innis; Catherine Cheng

doi:doi:10.18632/aging.206144

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Research Paper Volume 16, Issue 20 pp 13039—13075

Canonical ligand-dependent and non-canonical ligand-independent EphA2 signaling in the eye lens of wild-type, knockout, and aging mice

Figure 5. Canonically activated EphA2-pY589 protein is localized exclusively in epithelial cells. Longitudinal lens sections from 6-week-old, 4-month-old, and 8-month-old ephrin-A5^+/+ and ephrin-A5^-/- mice were stained with EphA2-pY589 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). The EphA2-pY589 fluorescence highlighted the lens epithelial cells (arrows) across all age-matched control and ephrin-A5^-/- lenses. Scale bar, 50 μm.