Research Paper Volume 16, Issue 20 pp 13039—13075
Canonical ligand-dependent and non-canonical ligand-independent EphA2 signaling in the eye lens of wild-type, knockout, and aging mice
Figure 4. In lens fiber cells, pan-EphA2 is localized to the cell membrane and enriched along the short sides. Lens sections in the cross orientation from 4-month-old control and ephrin-A5-/- mice were stained with pan-EphA2 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). E denotes the epithelial cells, and images of the fiber cells were taken in sequence along similar areas of the lens equator. Fluorescence from pan-EphA2 was along the cell membrane of peripheral and mature fiber cells in the lens cortex region (asterisks). The signal was enriched along the short sides of lens fiber cells. Scale bar, 20 μm.