Research Paper Volume 16, Issue 20 pp 13039—13075
Canonical ligand-dependent and non-canonical ligand-independent EphA2 signaling in the eye lens of wild-type, knockout, and aging mice
Figure 2. Pan-EphA2 is localized to epithelial and fiber cells with enriched signal in fiber cells. Longitudinal lens sections from 6-week-old, 4-month-old, and 8-month-old ephrin-A5+/+ and ephrin-A5-/- mice were stained with pan-EphA2 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). The pan-EphA2 signal was visible in epithelial cells (arrows) and was strongly localized within the cortical fiber cell region (asterisks). This pattern remained consistent between age-matched controls and ephrin-A5-/- lenses. Scale bar, 50 μm.