Research Paper Volume 16, Issue 20 pp 13039—13075
Canonical ligand-dependent and non-canonical ligand-independent EphA2 signaling in the eye lens of wild-type, knockout, and aging mice
Figure 10. In lens fiber cells, EphA2-pS898 signal is localized to the cell membrane of mature fibers. Lens sections in the cross orientation from 8-month-old control and ephrin-A5-/- mice were stained with EphA2-pS898 (green) antibody, phalloidin (F-actin, red), and DAPI (nuclei, blue). EphA2-pS898 signal was present in mature fiber cells (asterisks). The signal appeared cytosolic and did not have obvious membrane localization. The staining signals were comparable between control and ephrin-A5-/- sections. Scale bar, 20 μm.