Research Paper Volume 16, Issue 20 pp 13132—13144
OSBPL2 inhibition leads to apoptosis of cochlea hair cells in age-related hearing loss by inhibiting the AKT/FOXG1 signaling pathway
Figure 5. OSBPL2 deficiency inhibits the FOXG1 signaling pathway in H2O2-treated HEI-OC1 cells via AKT inactivation. (A) The levels of FOXG1-related proteins (FOXG1, WNT3A, and FOXO1) in HEI-OC1 cells from Control and H2O2 groups. (B) Co-IP assay for p-AKT and FOXG1 in HEI-OC1 cells. (C) FOXG1 protein level in HEI-OC1 cells transfected with Vector, FOXG1-OE, shNC or sh-FOXG1. (D) The levels of OSBPL2 and AKT/FOXG1-related proteins in H2O2, H2O2+MK2206, H2O2+MK2206+FOXG1-OE, H2O2+sh-OSBPL2, H2O2+sh-OSBPL2+SC79, and H2O2+sh-OSBPL2+SC79+sh-FOXG1 groups. All in vitro experiments were performed in triplicate (n = 3). * P<0.05; ** P<0.01.