OSBPL2 inhibition leads to apoptosis of cochlea hair cells in age-related hearing loss by inhibiting the AKT/FOXG1 signaling pathway

Meina Li-Yang; Chao Ma; Xiaoye Wang; Jianqiang You

doi:doi:10.18632/aging.206138

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Research Paper Volume 16, Issue 20 pp 13132—13144

OSBPL2 inhibition leads to apoptosis of cochlea hair cells in age-related hearing loss by inhibiting the AKT/FOXG1 signaling pathway

Figure 3. OSBPL2 deletion inactivates AKT signaling pathway. (A) The levels of AKT-related proteins (p-AKT, AKT, p-PI3K, PI3K, and PDK1) in HEI-OC1 cells from Control and H₂O₂ groups. (B) Co-IP assay for OSBPL2 and p-AKT in HEI-OC1 cells. (C) The levels of OSBPL2 and AKT-related proteins in HEI-OC1 cells from H₂O₂, H₂O₂+MK2206, H₂O₂+sh-OSBPL2, and H₂O₂+sh-OSBPL2+SC79 groups. All in vitro experiments were performed in triplicate (n = 3). * P<0.05; ** P<0.01.