OSBPL2 inhibition leads to apoptosis of cochlea hair cells in age-related hearing loss by inhibiting the AKT/FOXG1 signaling pathway

Meina Li-Yang; Chao Ma; Xiaoye Wang; Jianqiang You

doi:doi:10.18632/aging.206138

← Back

Research Paper Volume 16, Issue 20 pp 13132—13144

OSBPL2 inhibition leads to apoptosis of cochlea hair cells in age-related hearing loss by inhibiting the AKT/FOXG1 signaling pathway

Figure 2. OSBPL2 absence induces apoptosis in H₂O₂-treated HEI-OC1 cells. (A) OSBPL2 protein levels in HEI-OC1 cells transfected with shNC and sh-OSBPL2. (B) CCK-8 assay for the viability of HEI-OC1 cells from H₂O₂ and H₂O₂+sh-OSBPL2 groups. (C) TUNEL assay for the apoptosis of HEI-OC1 cells from each group. (D) Bax and Bcl-2 protein levels in HEI-OC1 cells from each group. All in vitro experiments were performed in triplicate (n = 3). * P<0.05; ** P<0.01.