Research Paper Volume 16, Issue 20 pp 12977—13011
Werner syndrome RECQ helicase participates in and directs maintenance of the protein complexes of constitutive heterochromatin in proliferating human cells
Figure 9. WRN facilitates transcription of the LBR gene. (A, B) RT-qPCR measurements of LMNB1 and LBR gene transcripts in the WI38hTERT expressing the indicated shRNAs (A) or in Δwrn-3 cells with or without WRN complementation (B). (C) A schematic of the 3’ end of the LBR gene with locations of the qPCR primers and an intronic R-loop forming site (RLFS). Exon numbers are below the gene. (D) RT-qPCR measurements of a ratio between the spliced LBR mRNA (EE, no intron 13) and the mRNA containing intron 13 (EI). The data are from WI38hTERT expressing the indicated shRNAs. Graphs in A, B, and D summarize three independent experiments each, with technical triplicates.