Research Paper Volume 16, Issue 20 pp 12977—13011

Figure 10. SATII satellite replication is affected by WRN deficiency in WI38hTERT. (A) A labeling scheme and an example of a HSATII array (green) with CldU replication tracks (red) within it, as visualized by fiber FISH/maRTA. Scale, 10 µm. Cells were labeled with CldU for 30 min and harvested after a 30 min chase in fresh media without label. (B) Length distributions of HSATII arrays that do not (non-replicating, nr) or do (replicating, r) carry CldU tracks within them. The length distribution of CldU tracks in total genomic DNA (lane 5), is included for comparison. (C) Length distributions of CldU tracks in the genome (C_gen) and within HSATII arrays (C_SAT). The plots are representative of two independent experiments. See Methods for the explanation of p-value calculations. Crossbars are distribution medians. (D) Size of difference between the indicated CldU track length distributions in the control and WRN-depleted cells were determined by deriving Cliff’s delta statistic. Black dots are independent experiments and open triangles are means of the two experiments. The Cliff’s delta values derived from the experiment shown in (B) are marked by arrows. Smaller Cliff’s delta values indicate smaller difference between C_gen and C_SAT.