Research Paper Volume 16, Issue 19 pp 12928—12951

Figure 4. ABCA1 mediates the effects of GATA2 downregulation on macrophages by regulating inflammation and ingestion. RAW264.7 cells were incubated with or without siGata2 for 48 h, and they were then treated with or without Falcarindiol for another 24 h. mRNA levels of Gata2, Abca1, Pacsin1, Tlr4, Myd88, Megf10, and Gulp1 were assessed by qPCR (A) and their protein levels, Flotillin1 was added, were measured by western blotting (B) and immunofluorescence staining (C) in RAW264.7 cells from each group. mRNA levels of Cd80, Cxcl1, Ccl2, Il23, Cxcl10, Cxcl11, Cxcl12, Mmp9, Mmp12, Tgfb1, Cd163, Cd209, Marco, Stab2, Sirpa, Lamp2 and Gpnmb were measured by qPCR (D, E) in RAW264.7 cells from each group (siNC, black bars; siGata2-1, gray bars; siGata2-2, white bars; siGata2-1+Falcarindiol, purple bars; siGata2-2+Falcarindiol, cyan bars). Phagocytosis assessment was done by Cell Meter^™ Fluorimetric Phagocytosis Assay Kit (F). Scale bars = 50 μm. ^*P < 0.05 or ^**P < 0.01 vs. siNC and ^#P < 0.05 or ^##P < 0.01 vs. siGata2.