Research Paper Volume 16, Issue 19 pp 12928—12951

Figure 1. GATA2 is downregulated in pulmonary macrophages during the development of CS-triggered COPD. mRNA levels of GATA2 were analyzed in database GSE171541 and CSE173896 (A). Eight-week-old mice were exposed to cigarette smoke (CS) or room air (n = 5–10). The lungs were harvested 2, 3, and 4 months after CS exposure and subjected to immunofluorescence staining of F4/80 and GATA2 (B). Scale bars = 50/100 μm. Relative integrated density of GATA2 fluorescence in F4/80 marked cells was measured from each group (Air, round dots; CS, triangle dots). mRNA and protein levels of GATA2 were measured by qPCR, western blotting, and immunofluorescence staining (C) in RAW264.7 cells treated with CSE for 24 h from each group (CSE-0%, blue bars; CSE-2.5%, magenta bars; CSE-5%, green bars; CSE-10%, orange bars). ^*P < 0.05 or ^**P < 0.01 vs. Air 5–10 animals per group or CSE-0%.