Research Paper Volume 16, Issue 19 pp 12697—12725

Figure 7. CB3 effects on inflammatory phenotype in aged macrophages in vitro. Macrophages are chronically treated with 100uM CB3 for up to 14 days. (A) RT-qPCR analysis for SASP markers MCP-1, IL-6, IL-1b and TNF-a transcripts normalized to 36B4 (n=7). (B) MCP-1, IL-1β, IL-6, TNF-α, KC-GRO, IL-10 and MMP-9 protein levels assessed by MSD multiplex on conditioned media harvested 24h after serum derivation (n=6). Error bars represent the mean ± SEM. p-values were obtained comparing groups overtime using a non-parametric one-way ANOVA analysis (Kruskal-Wallis analysis followed by Dunnett’s multiple comparison test; *p<0.05; **p<0.01; ***p<0.001) or using a non-parametric t-test (Mann-Whitney) to analyze the significance between groups treated or not treated with CB3 (#p<0.05; ##p<0,01, ###p<0.001).