Research Paper Volume 16, Issue 19 pp 12697—12725

Figure 6. CB3 effects on proliferation and apoptosis in aged macrophages in vitro. Macrophages are chronically treated with 100uM CB3 for up to 14 days. (A) RT-qPCR analysis for p21^CIP1 transcripts normalized to 36B4 (n=7). (B) Immunoblot and p21^CIP1 quantification by densitometric analysis (n=3). (C) Quantification of EdU-positive cells. EdU was added for 24 h before analysis. (D) Quantification of TUNEL positive cells (n=3). Error bars represent the mean ± SEM. p-values were obtained comparing groups overtime using a non-parametric one-way ANOVA analysis (Kruskal-Wallis analysis followed by Dunnett’s multiple comparison test; *p<0.05; ***p<0.001) or using a non-parametric t-test (Mann-Whitney) to analyze the significance between groups treated or not treated with CB3 (#p<0.05).