Research Paper Volume 16, Issue 19 pp 12697—12725

Figure 5. Functional alterations in aged macrophages in vitro. (A) Gating strategy for flow cytometry analysis of phagocytosis after 3h of pHrodo Red zymosan treatment. Right upper panel: Quantification of the number of phagocytic macrophages (% of positive cells). Right below panel: Quantification of the mean fluorescence intensity (MFI) representing phagocytosis capacity (n=3). (B) Macrophages are incubated with LPS (10 ng/mL) for 24h. Quantification of the pro-inflammatory response to LPS by RT-qPCR by measuring the relative expression of MCP-1 and IL-6 mRNAs. Fold induction is expressed between the control and the LPS (n=7). Error bars represent the mean±SEM. p-values were obtained comparing groups overtime using a non-parametric one-way ANOVA analysis (Kruskal-Wallis analysis followed by Dunnett’s multiple comparison test; *p<0.05; **p<0.01).