Research Paper Volume 16, Issue 19 pp 12697—12725
A new model and precious tool to study molecular mechanisms of macrophage aging
Figure 4. Metabolic phenotype in aged macrophages in vitro. (A) Seahorse analysis of oxygen consumption rate (OCR), basal and maximal respiration. Results are normalized by cell count using DAPI staining (n=3). (B) Seahorse analysis of extracellular acidification rate (ECAR), basal glycolysis and glycolytic capacity. Results are normalized by cell count using DAPI staining (n=8). Error bars represent the mean ± SEM. p-values were obtained comparing groups overtime using a non-parametric one-way ANOVA analysis (Kruskal-Wallis analysis followed by Dunnett’s multiple comparison test; *p<0.05; **p<0.01).