Research Paper Volume 16, Issue 19 pp 12697—12725

Figure 3. SASP analysis in aged macrophages in vitro. Murine peritoneal macrophages from young mice (3 months) were cultured during 2, 7 or 14 days in vitro. (A) RT-qPCR analysis for SASP markers MCP-1, IL-6, TNF-a and IL-1b transcripts normalized to 36B4 (n=13). (B) MCP-1, IL-1β, IL-6, TNF-α, KC-GRO, IL-10 and MMP-9 protein levels assessed by MSD multiplex on conditioned media harvested 24h after serum deprivation (n=4-7). Error bars represent the mean ± SEM. p-values were obtained comparing groups overtime using a non-parametric one-way ANOVA analysis (Kruskal-Wallis analysis followed by Dunnett’s multiple comparison test; *p<0.05; **p<0.01; ***p<0.001; ****p<0.0001).