Research Paper Volume 16, Issue 19 pp 12697—12725

Figure 2. Proliferation and apoptosis of aged macrophages in vitro. Murine peritoneal macrophages from young mice (3 months) were cultured during 2, 7 or 14 days in vitro. (A) Left panel: EdU incorporation assay (scale bar 100 μm). Right panel: quantification of EdU-positive cells. EdU was added for 24 h before analysis (n=9). (B) Left panel: DNA fragmentation detected by TUNEL. Apoptotic cells were visualized as green, and the nuclei as blue (scale bar 100 μm). Right panel: quantification of TUNEL positive cells (n=3). Error bars represent the mean ± SEM. p-values were obtained comparing groups overtime using a non-parametric one-way ANOVA analysis (Kruskal-Wallis analysis followed by Dunnett’s multiple comparison test; *p<0.05; ***p<0.001).