Research Paper Volume 16, Issue 18 pp 12651—12666

Figure 3. Result of RNA-sequencing analysis of reversibly immortalized NHEK with Dox induction or PBS control. (A) Principal component analysis (PCA) plot of gene expression of biological triplicates from RNA-seq of reversibly immortalized NHEK with PBS or Dox treatment. (B) Volcano plot of up- or downregulated genes in reversibly immortalized NHEK with PBS or Dox treatment with top-ranked genes annotated. (C) Results of Gene Sets Enrichment Analysis (GSEA) of the Hallmark gene sets for DEGs between PBS and Dox group, with red bars showing enriched terms in upregulated genes and blue bars showing enriched terms in downregulated genes. (D) Bubble plot of GO enrichment analysis of upregulated DEGs. (E) Bubble plot of KEGG pathway enrichment analysis of upregulated DEGs. (F) Bubble plot of GO enrichment analysis of downregulated DEGs. (G) Bubble plot of KEGG pathway enrichment analysis of downregulated DEGs.