Research Paper Volume 16, Issue 19 pp 12866—12892

Figure 5. FAM3D expression is inversely associated with SESN2 expression. (A) Volcano plot and (B) hierarchical clustering heatmap showing DEGs between FAM3D KO and NC LoVo cells. (C) GO functional analysis showed the top 30 biological processes of significantly upregulated genes after FAM3D KO. (D) KEGG enrichment analysis of genes significantly upregulated after FAM3D KO. (E, F) RT-qPCR was used to detect changes in the expression of representative genes in the p53 and mTOR pathways in FAM3D (E) KO or (F) overexpressing LoVo cells. (G, H) The mRNA expression of SESN2 in normal tissues and CRC tissues in (G) GSE18105 and (H) GSE106582. (I, J) Kaplan-Meier curves of DFS in (I) GSE161158 and (J) TCGA. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.