Research Paper Volume 16, Issue 19 pp 12866—12892

Figure 2. FAM3D promotes CRC cell proliferation, migration, and invasion in vitro. (A) The localization of FAM3D in LoVo and HT29 cells. (B) Schematic diagram of sgRNA targeting the human FAM3D gene locus. Two sgRNA sequences, sgRNA1 and sgRNA2, are marked in red, and the protospacer adjacent motif (PAM) sequences are presented in blue. (C) Western blot analysis of FAM3D in the supernatant (secreted) and cell lysate (lysate) of LoVo and HT29 cells after FAM3D KO. (D–F) Cell proliferation was detected by (D) colony formation assay and (E, F) CCK-8 assay in LoVo and HT29 cells. (G, H) Cell migration was detected by a wound healing assay in (G) LoVo and (H) HT29 cells. **P < 0.01, ***P < 0.001, ****P < 0.0001.