Research Paper Volume 16, Issue 19 pp 12866—12892

Figure 1. Methylation, expression and prognosis of FAM3D. (A, B) Correlations between FAM3D mRNA expression and methylation of the FAM3D promoter in the (A) TCGA and (B) GEO databases (GSE106582 + GSE101764). (C, D) Kaplan-Meier curves of (C) OS and (D) DFS based on the methylation of the FAM3D promoter in tumor tissues from the TCGA cohort. (E, F) Relative FAM3D mRNA and protein expression after treatment with a series of concentrations of 5-Aza in (E) CW2 and (F) LS513 cells (differences compared to the DMSO group). (G) The mRNA expression of FAM3D in normal tissues and CRC tissues in the TCGA cohort. (H) The mRNA expression of FAM3D in normal cells and CRC cells in GSE132465. (I) The protein expression of FAM3D in normal tissues and COAD tissues in the CPTAC cohort. (J) Kaplan-Meier curve of OS based on FAM3D mRNA expression in tumor tissues from the TCGA cohort. *P < 0.05; **P < 0.01, ***P < 0.001, ****P < 0.0001.