Research Paper Volume 16, Issue 19 pp 12850—12865

Figure 2. Correlations between USP4 expression and clinical features of PTC patients. (A) Violin plot depicting USP4 gene expression levels in PTC and adjacent normal thyroid tissues sourced from the TCGA database; (B) Comparison of USP4 mRNA expression levels across different N stages in PTC tissues, as sourced from the TCGA database; (C) Kaplan-Meier curve estimating overall survival rates of PTC patients from the TCGA database, stratified by varying USP4 expression levels; (D) Representative IHC staining images showing USP4 expression in PTCs and their corresponding normal tissues; (E) Statistical quantification of IHC scores for USP4 expression in PTCs and their corresponding normal tissues; (F) Representative IHC images showing weak, moderate, and strong staining of USP4 in PTCs; (G) Univariate analysis examining the associations between USP4 protein levels and clinicopathologic features in PTC patients from the validation cohort. Red lines denote clinicopathologic features significantly associated with USP4 expression. (H, I) Kaplan-Meier curves for overall survival (OS) and recurrence-free survival (RFS) illustrating PTC patient outcomes in relation to high versus low USP4 expression levels. All ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001.