Figure 2.Immune microenvironment in COPD induced by cigarette smoke. (A, B) Mass cytometry was utilized to examine alterations in immune cell composition in the lung tissue of the COPD C57BL/6 mouse model (n = 5 replicates). (C, D) Moreover, flow cytometry analysis revealed a significant rise in immune cells in the lung tissue of COPD mice. Data are shown as the mean ± SEM (n = 3-5 replicates). *P < 0.05; **P < 0.01; *** P < 0.001; ****P < 0.0001.