Research Paper Volume 16, Issue 18 pp 12543—12558

Figure 5. Effects of DDIT3 on Wnt/β-catenin signaling affected by BMP9 in C3H10T1/2 cells. (A) Confocal assay of immunofluorescent staining results shows the effect of DDIT3 on nucleus location of β-catenin induced by BMP9. (B) Western blot assay results show the effect of DDIT3 on the protein level of β-catenin affected by BMP9. (C) Quantitative results of Western blot assay show the effect of DDIT3 on the protein level of β-catenin affected by BMP9. (D) Western blot assay results show the effect of DDIT3 on phosphorylation of GSK-3β (p-GSK-3β) and total GSK-3β induced by BMP9. (E) Quantitative results of Western blot assay show the effect of DDIT3 knockdown on the ratio of p-GSK-3β to GSK-3β affected by BMP9. (F) Western blot assay results show the effect of DDIT3 knockdown on the level β-catenin affected by BMP9. (G) Quantitative results of Western blot assay show the effect of DDIT3 knockdown on the level β-catenin affected by BMP9. (H) Western blot assay results show the effect of DDIT3 knockdown on the protein level and phosphorylation of GSK-3β affected by BMP9. (I) Quantitative results of Western blot assay show the effect of DDIT3 knockdown on the ratio of p-GSK-3β to GSK-3β affected by BMP9. “*” p <0.05, “**” p <0.01, compared with the BMP9 group; “#” p<0.05, “##” p<0.01. All data were repeated in three independent experiments.