Research Paper Volume 16, Issue 17 pp 12277—12292

Figure 6. FOSL1 increased CIB2 expression levels at the transcriptional level. (A) Venn diagram showing overlap between RNA sequence data (PC-9 vs. PC-9G) and the CIB2 upstream transcription factor predicted by two websites: JASPAR (http://jaspar.genereg.net/) and Gene Cards (https://genecards.com/). (B) Relative mRNA expression levels of FOSL1 were detected by qRT-PCR in PC-9 and PC-9G cells. (C, D) The FOSL1 and CIB2 expression levels were detected in FOSL1-overexpressed cells by qRT-PCR in PC-9 cells. (E) The WT/MUT binding sites and FOSL1 motif in the promoter region of CIB2 were shown. (F) The luciferase reporter assays were used to test CIB2-WT or CIB2- MUT binding activities with FOSL1. (G) Correlation relationships between CIB2 and ZEB1 were analyzed in the cBioPortal database (https://www.cbioportal.org/). (H) Cistrome database was used to show the binding peaks of FOSL1 at CIB2 promoter region (http://cistrome.org/db/#/). Data were statistically analyzed using Student’s t-test and values were shown as mean ± SD of three independent experiments. ^*indicated significant difference at p < 0.05, ^*** indicated significant difference at p < 0.001.