Research Paper Volume 16, Issue 17 pp 12277—12292

Figure 5. ZEB1 functioned as a downstream regulator in CIB2-induced EMT and chemoresistance. (A) PC-9 cells with stably overexpressed CIB2 were transfected with shZEB1#1 or shZEB1#2 for 48 hours. Western blotting was used to detect the protein expression levels of ZEB1 and CIB2. (B, C) Cell migration and invasion abilities were measured after silencing ZEB1 in CIB2-overexpressed stable PC-9 cells. Quantitative analysis of migration and invasion cell numbers were shown in histogram. (D–F) PC-9G cells with CIB2 knockout were transfected with ZEB1 overexpression plasmid. Cell migration and invasion abilities were measured and analyzed. Data were statistically analyzed using Student’s t-test and values were shown as mean ± SD of three independent experiments. ^**indicated significant difference at p < 0.01.