Research Paper Volume 16, Issue 17 pp 12277—12292

Figure 4. CIB2 increases the expression levels of ZEB1, EMT-associated marker to promote gefitinib resistance. (A) Morphological changes were tracked by microscopy in CIB2-overexpressed cells. Scale bar = 100 μm (×400 magnification). (B) GSEA program was used to analyze HALLMARK_Epithelial–Mesenchymal Transition pathway enrichment scores between CIB2 high- and low-expression groups using TCGA lung cancer dataset. (C) The expression of N-Cadherin, Vimentin, E-Cadherin, and ZEB1 was determined using Western blotting. (D) Correlation analysis of the TCGA dataset to assess expression of CIB2 and ZEB1 in lung cancer tissue using the GEPIA database (http://gepia.cancer-pku.cn/index.html). (E) The correlation analysis of the ZEB1 expression and HALLMARK_Epithelial Mesenchymal Transition pathway in lung cancer tissues (https://bio.tools/emtome). (F, G) Immunofluorescence assay (IF) was used to detect E-cadherin and Vimentin levels in CIB2 knockout stable cells compared to control cells.