Research Paper Volume 16, Issue 17 pp 12277—12292

Figure 2. CIB2 increases gefitinib resistance by inhibiting cellular apoptosis. (A) CIB2 knockout stable cell lines were constructed by lentivirus infection, and the expression levels of CIB2 were analyzed by Western blotting. (B) The gefitinib sensitivities were analyzed in CIB2 knockout cells treated with different concentrations of gefitinib for 72 h using CCK8 kit. (C) CIB2 knockout cells were treated with different gefitinib or PBS for 48 h, then the proportion of cell apoptosis were measured by flow cytometry. (D) Cell apoptosis rates were quantitatively calculated by histogram. (E) The protein expression levels of BAX, Caspase3 and Caspase8 were detected by Western blotting in cells with CIB2 knockout. Data were statistically analyzed using Student’s t-test and values were presented as mean ± SD of three independent experiments. ^*indicates significant difference at p < 0.05.