Research Paper Volume 16, Issue 17 pp 12277—12292

Figure 1. CIB2 expression was dramatically increased in gefitinib-resistant cells and higher CIB2 levels in lung cancer patients were linked to poor prognosis. (A) Heat map of RNA-seq results (PC-9G vs. PC-9). (B, C) CIB2 mRNA and protein expression levels were detected by Western blotting and qRT-PCR in PC-9 and PC-9G cells. (D) The CIB2 expression levels were analyzed and compared in tumor and normal tissues of 22 human cancers by pan-cancer analysis through TNM plot (https://tnmplot.com/analysis/). Significant differences by Mann-Whitney U-test are marked with red. (E) The protein expression of CIB2 in normal and tumor samples obtained from the Biobank. GAPDH levels were used as an internal loading control. (F, G) CIB2 expression in patients of LUAD (normal = 52, tumor = 52) and LUSC (normal = 59, tumor =515) were examined in TCGA database. (H) Kaplan-Meier plot of the overall survival of patients with lung cancer with high or low expression of CIB2 from Kaplan-Meier plotter (http://kmplot.com/analysis/). Data were statistically analyzed using Student’s t-test and values were presented as mean ± SD. ^***indicated significant difference at p < 0.001.