Research Paper Volume 16, Issue 17 pp 12263—12276
Excessive glucocorticoids combined with RANKL promote the differentiation of bone marrow macrophages (BMM) into osteoclasts and accelerate the progression of osteoporosis by activating the SYK/SHP2/NF-κB signaling pathway
Figure 3. Role of the SYK/SHP2/NF-κB signaling pathway in osteoporosis. (A, B) Western blot analysis of primary cultured BMM cells treated with inhibitors targeting glucocorticoid receptor (GR), SYK, or SHP2 kinase domains (SHP2-KD) was performed. Inhibition of GR, SYK, or SHP2 resulted in decreased phosphorylation levels of NF-κB, SYK, SHP2, JAK2, and TAK1. Notably, SHP2-KD led to reduced p-SHP2, t-SHP2, p-JAK2, and p-TAK1 levels compared to SYK inhibition, suggesting SHP2’s downstream role in the pathway. **P < 0.01; nsP > 0.05.