Research Paper Volume 16, Issue 17 pp 12263—12276
Excessive glucocorticoids combined with RANKL promote the differentiation of bone marrow macrophages (BMM) into osteoclasts and accelerate the progression of osteoporosis by activating the SYK/SHP2/NF-κB signaling pathway
Figure 2. Impact of macrophage-specific SHP2 knockout on kinase activity and transcription factors in ovariectomized mice. (A, B) Western blot analysis was performed on tissue samples to evaluate the protein expression levels of phosphorylated NF-κB (p-NF-κB), spleen tyrosine kinase (p-SYK), and total and phosphorylated SHP2 (t-SHP2 and p-SHP2, respectively). SOP mice showed elevated levels of these proteins compared to non-SOP mice. In contrast, SOP mice with macrophage-specific SHP2 knockdown exhibited reduced levels of p-NF-κB, p-SHP2, and t-SHP2, with unchanged p-SYK levels. **P < 0.01; nsP > 0.05.