Research Paper Volume 16, Issue 16 pp 11796—11808
2-Bromopalmitate treatment attenuates senescence phenotype in human adult cells - possible role of palmitoylation
Figure 4. The influence of 2-BP treatment on the levels of senescence markers. The levels of p-p53 (A), HMGB1 (B), and lamin B1 (C) in cell extracts from VSMCs treated for 24 h with 2-BP before doxorubicin (DOX) treatment (pretreatment) or treated with 2-BP alone for the indicated times were analyzed via Western blotting. Representative Western blots (the cutting line of the blot is shown in red) (D). The results are presented as the means ± SEMs from N=3 biological replicates. Statistical analysis was performed using one-way ANOVA; *p< 0.05, **p<0.01, ***p<0.001.