Research Paper Volume 16, Issue 16 pp 11796—11808

Figure 1. The influence of 2-BP treatment on cell proliferation and senescence. The graph shows the total protein palmitoylation normalized to the control after 24 h of 2-BP treatment (A). Analysis of the effects of different doses of 2-BP on the proliferation rate, as measured by growth rate calculations (B) and recording of cell numbers during a 4-day culture (C). Changes in the number of BrdU-positive cells (D), the level of SA-β-gal activity (E), and the number of DNA damage-associated 53BP1 foci (F) and γH2AX foci (G). Representative images of the measurements are shown in Panels (H) (BrdU incorporation) and (I) (SA-β-gal activity, 53BP1, γH2AX). The results are presented as the means ± SEMs from N=3 biological replicates. Statistical analysis was performed using one-way ANOVA or two-way ANOVA for (C), *p< 0.05, **p<0.01, ****p<0.0001.