Research Paper Volume 16, Issue 17 pp 12252—12262

Figure 5. PARK7 functioned as a downstream molecule under EFCAB7. (A). RT-PCR analysis of the overexpression efficiency of PARK7 in EFCAB7 knockdown Hep3B cell line; (B). RT-PCR analysis of EFCAB7 mRNA expression level after the overexpression of PARK7 in Hep3B cell line; (C). Proliferation of stable transfected Hep3B tumor cells was measured by absolute cell counting (n=3); (D). Representative images of treated Hep3B in the same fields after 1, 2, 3, 4 and 5 days. (E). MTT assay analyzed the growth of stable transfected Hep3B tumor cells in different groups; (F) and (G). Transwell assays were conducted to measure the migration abilities of different treated tumor cells; (H). Expression of PARK7 in TCGA database; (I). RT-PCR analysis of PARK7 in HCC tumor tissues and adjacent tissues before and after RFA. Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001).