Research Paper Volume 16, Issue 17 pp 12239—12251
MTA2 knockdown suppresses human osteosarcoma metastasis by inhibiting uPA expression
Figure 2. Knockdown of MTA2 inhibits human osteosarcoma cell migration and invasion. (A, B) Stable MTA2 knockdown in HOS and 143B cells was ascertained by western blot and RT-qPCR assay. (C) Cell growth ability of shLuc- and shMTA2- osteosarcoma cells by MTT assay. (D) Proliferation rates of shLuc- and shMTA2- osteosarcoma cells as assessed by colony formation assay. (E) In vitro migration and invasion assays to evaluate the effect of MTA2 knockdown on the migratory and invasive abilities of HOS and 143B cells. ** P < 0.01.