Research Paper Volume 16, Issue 17 pp 12239—12251

Figure 1. MTA2 overexpression in human osteosarcoma tissue and osteosarcoma cell lines is associated with poor prognosis in osteosarcoma patients. (A) Representative immunohistochemistry (IHC) results of MTA2 staining in normal and osteosarcoma specimens. Low: low expression of MTA2; High: high expression of MTA2. Scale bar: 50 μm. (B) Representative IHC results of MTA2 staining in human osteosarcoma specimens of different tumor stages. (C) Comparison of MTA2 expression between normal and tumor tissues from osteosarcoma patients using TNMplot from the TCGA database. (D) Kaplan–Meier analysis of overall survival in osteosarcoma patients with high and low MTA2 expression levels. (E, F) Protein and mRNA expression of MTA2 in 4 human osteosarcoma cell lines, as assessed using western blot and RT-qPCR assays. ** P < 0.01; # P < 0.05.