Research Paper Volume 16, Issue 16 pp 11926—11938
Caffeic acid derivative MPMCA suppresses osteoclastogenesis and facilitates osteoclast apoptosis: implications for the treatment of bone loss disorders
Figure 5. MPMCA enhances the level of cleaved caspase-3 in mature osteoclasts. (A) Immunofluorescence staining was performed on RAW 264.7 cells to detect cleaved caspase-3. The cells were initially stimulated with RANKL for 5 days, and then treated with RANKL and MPMCA for an additional 2 days. Cleaved caspase-3 is indicated by green, while the staining for nuclei using DAPI is shown in blue. (B) The enumeration of cells that had positive staining was conducted (n = 3). *p < 0.05 vs. control group. #p < 0.05 vs. RANKL-treated group.