Research Paper Volume 16, Issue 16 pp 11893—11903
Notch1 signaling pathway promotes growth and metastasis of gastric cancer via modulating CDH5
Figure 4. Notch1 positively regulated the expression of CDH5. (A) Notch1 positively regulated mRNA levels of CDH5 in MGC-803 (upper) and HGC-27 cells (lower). (B) Notch1 positively regulated protein levels of CDH5 in MGC-803 (upper) and HGC-27 cells (lower). (C) Schematic diagram of putative binding sites (−579 to −570) within the CDH5 promoter. (D) CHIP assay showed Notch1 bind to the CDH5 promoter. Input - input fraction, IgG - sample incubated with rabbit IgG, Notch1 Ab – sample incubated with anti-Notch1 antibody.